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CRISPR gene editing has become a cornerstone technology in modern life science research, enabling precise modifications in genomes across a wide range of organisms. However, successful gene editing does not end with the editing step itself. Researchers must verify whether the intended edit has occurred accurately and efficiently. This makes CRISPR validation a critical component of every genome editing workflow.

Sanger sequencing, often referred to as the chain-termination method, remains one of the most trusted and widely used DNA sequencing technologies in modern molecular biology. Developed by Frederick Sanger in 1977, it delivers exceptional accuracy and clear,