Bulk RNA sequencing is an essential tool for transcriptome-wide studies, offering valuable insights into differential gene expression, alternative splicing, allele-specific expression, and time-series experiments. This technique enables a deep analysis of gene activity and transcriptional variations across conditions.
Our library preparation process begins with RNA as the starting material, employing Poly-A enrichment to selectively isolate mRNA. This ensures the highest-quality sequencing data, enabling precise and reliable gene expression profiling.
What Bulk RNA-seq is used for ?
| Differential Gene Expression | Transcriptome-wide Expression Profiling | Cost-effective, High-throughput Studies | ||
• Condition vs control comparisons • Drug or perturbation response • studies Time-course experiments | • Gene-level expression quantification • Pathway and functional enrichment analysis • Sample clustering and global expression patterns | • Large cohorts and biological replicates • Screening and pilot experiments • Projects requiring fast turnaround at low cost |
Multiple starting materials available, with optional rRNA depletion or mRNA enrichment during library preparation. High-quality QC and a variety of selectable data outputs.
You may provide purified RNA, or we can perform RNA extraction as the starting material.
mRNA enrichment/rRNA depletion → library preparation → library QC
FASTQ delivery starting from 20M reads per sample.
Differential gene expression analysis.
| Sequencing Depth | Turnaround Time | Add-on | Library Preparation |
| 20M SE (40M PE) Reads | 5-10 Business Days | • Deeper sequencing • Additional customized analysis | mRNA enrichment OR rRNA depletion |
| 25M SE (50M PE) Reads | 5-10 Business Days | ||
| 30M SE (60M PE) Reads | 5-10 Business Days | ||
| 40M SE (80M PE) Reads | 5-10 Business Days |
mRNA-Seq | Total RNA-Seq | ||
Target RNA | mRNA | mRNA+IncRNA | |
Designed For | Gene expression profiling with a focus on coding RNA | Gene expression profiling including non-coding RNA | |
RNA Selection Method | Poly(A) selection | rRNA depletion | |
Starting Material | • Fresh, frozen or fixed/FFPE • Total RNA, cells, tissue | • Fresh, frozen or fixed/FFPE • Total RNA, cells, tissue | |
Principles |  |  |
Standard Sample Type
• Mouse tissue
• Cell pellets
• Purified RNA
Standard Volume
• 50 μL RNA at 100 ng/ μL, RIN＞7
• 250,000 cells
• 1 gram tissue
FASTQ Files
• Per-sample FASTQ output
• 20M- 40M SE reads per sample
Data Analysis
• QC report
• Differential gene expression analysis
• GOI analysis
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