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Sanger Sequencing
Sanger Sequencing

Overnight Turnaround, Starting at Just $4

Home Services DNA Reading Sanger Sequencing
Overview

Accelerate your research with Quintara’s Sanger Sequencing services. We specialize in delivering accurate and reliable DNA sequencing results with an industry-leading overnight turnaround. Our service is designed for efficiency and supports a wide range of applications, including plasmid verification and mutation analysis, ensuring that your projects move forward swiftly without sacrificing quality.

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Services
NameDescriptionTurnaroundPrice
Plasmid VerificationSamples can be mixed or non-mixed with primers1 dayStarting from $4
Direct Colony SequencingWe use PCR technology1 dayStarting from $7
* We offer free universal primers, or we can synthesize your primers in-house
Sample Submission Guideline
10- 15 μL Plasmid DNA
1. Premix Primer with Plasmid DNA Sample
2. Plasmid DNA and Primer in Separate Tubes, or Use Our Free Primer Library.  (Complete List in Excel or Search Online)
Plasmid DNA Template5 μL Primer1
VolumeConcentrationConcentration
10- 15 μL80 ng/ μL5 pmol/μL (5 μM)
1Primer For Sequencing: Prefer Tm: 50-60℃, GC content: 40-60%
Purified or Unpurified1, ~10 ng of purified PCR per Kb
1. Premix Primer with Purified PCR DNA Sample

2. Purified/Unpurified PCR DNA and Primer in Separate Tubes, or Use Our Free Primer Library.  (Complete List in Excel or Search Online)

3. Please provide the PCR product size information at the time of order submission

Purified PCR5 μL Primer2
Type & SizeConcentrationConcentration
< 1 Kb10 ng/ μL5 pmol/μL (5 μM)
1 kb – 2 kb20 ng/ μL
2 kb – 3 kb30 ng/ μL
3 kb – 4 kb40 ng/ μL
>4kb

1In the case of unpurified PCR samples, we will conduct a PCR purification process prior to sequencing

2Primer For Sequencing: Prefer Tm: 50-60℃, GC content: 40-60%

agar plates, colony suspensions or overnight cultures


DNA Amplification Methods:

1. PCR (Preferred): This method allows for next-day data delivery. Please provide the amplicon size, as well as forward and reverse PCR primers.

2. Rolling Circle Amplification (RCA).

3. Plasmid Miniprep: Include antibiotic information for the sample.




Note: RCA and plasmid miniprep methods may extend the data delivery timeline by an additional day.          

          Ensure all suspension samples are placed in either tightly sealed 8-strip tubes or in a strip-capped 96-well plate


1. PCR amplification prior to sequencing. Please provide the PCR primer and the Expected Amplicon Size.


2. A minimum of 10 μL DNA Sample with a concentration of at least 30 mg/μL.





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