Choosing the Right WPS Approach

• Scenario Description

This method works well for plasmids smaller than 10 kb, with typical single continuous readable target fragments up to 1–3 kb; it prioritizes localized sequence validation rather than complete circular plasmid characterization.

• Recommended Service: Sanger Sequencing

The gold standard method for validating short DNA sequences, offering unmatched precision for your specific fragment needs. 

Standard Deliverables: AB1 chromatogram files, FASTA sequence files, comprehensive base quality reports, and professional mutation annotation analysis.

• Ultra-High Accuracy

  Regarded as the gold standard for short fragments, it is perfect for confirming point mutations and small insertions/deletions (indels) with high confidence.

• Cost-Effective Solution

  Eliminates the expense of full-plasmid sequencing, making it a budget-friendly choice for verifying local fragments and specific genetic regions.

• Minimal Sample Requirements

  Accepts a variety of sample types including purified plasmid DNA, bacterial colony suspensions, and overnight liquid cultures for added convenience.

• Scenario Description

Targeting plasmids of up to 25 kb with complex architectures—including tandem repeats, high-GC content, and viral vector sequences (ITR/LTR). This scenario demands rigorous confirmation of directional cloning accuracy, open reading frame (ORF) integrity, and structural consistency across challenging genomic regions.

• Recommended Service: Nanopore WPS

The gold standard for unambiguous assembly. 

Standard Deliverables: FASTA/GenBank sequence files,  and interactive quality control (QC) tool access.

• Primer-Free Full-Length

  A single ultra-long read spans the entire plasmid, eliminating assembly gaps and ensuring complete coverage of all genetic elements.

• PCR-Free & Bias-Free

  Eliminates PCR amplification bias entirely, preserving the true epigenetic state and native sequence representation of the plasmid.

• Unlimited De Novo Assembly

  Performs de novo assembly without reliance on pre-designed primers, ideal for unknown or highly modified plasmid structures.

• Scenario Description

Emergency QC before cell transfection or post-editing rapid verification demands immediate results. Our service is engineered to deliver actionable insights within hours, keeping critical experiments on track.

• Recommended Service: Nanopore WPS

Ideal for time-critical workflows requiring overnight or same-day turnaround for high-confidence results. 

Standard Deliverables for Urgent Requests: We provide a concise Quick QC Report summarizing key metrics, plus the complete full sequence file for downstream analysis—all delivered overnight or same-day.

• Real-time Data Output

  Deliver data overnight or same-day, works for time-critical projects.

• Simplified Prep

  No complex purification needed. Directly accepts crude samples like glycerol stocks and bacterial cultures.

• Scenario Description

Emergency QC before cell transfection or post-editing rapid verification demands immediate results. Our service is engineered to deliver actionable insights within hours, keeping critical experiments on track.

• Recommended Service: Nanopore WPS

Ideal for time-critical workflows requiring overnight or same-day turnaround for high-confidence results. 

Standard Deliverables for Urgent Requests: We provide a concise Quick QC Report summarizing key metrics, plus the complete full sequence file for downstream analysis—all delivered overnight or same-day.

• Real-time Data Output

  Deliver data overnight or same-day, works for time-critical projects.

• Simplified Prep

  No complex purification needed. Directly accepts crude samples like glycerol stocks and bacterial cultures.

• Scenario Description

For plasmids and viral vectors used in gene therapy, the verification process demands not only the complete structural characterization of the construct but also single-base high accuracy to meet the strict safety and efficacy standards of clinical applications.

• Recommended Service: Nanopore + Illumina Hybrid Sequencing

A synergistic approach combining the strengths of long-read and short-read sequencing technologies for uncompromising results. 

Standard Deliverables: Integrated high-precision full-length consensus sequence, cross-verification quality control report, and detailed structural annotation analysis.

• Long Reads for Full Structure

  Nanopore sequencing reconstructs the full-length backbone and resolves complex genomic regions, such as repeats and GC-rich areas, that short reads alone cannot clarify.

• Short Reads for Base Accuracy

  Illumina sequencing corrects random base errors from long reads, ensuring the final consensus sequence achieves Q30+ accuracy, critical for single-base variant detection.

• Integrated Top-Tier Data

  Advanced bioinformatics pipelines merge data from both platforms to deliver a unified, high-precision report that satisfies the rigor of clinical and regulatory submissions.