• We use your colony/bacterial sample to grow a culture followed by a miniprep prior to sequencing. Therefore, colony samples typically have a 2-day turnaround time.

• While sequencing of colony samples using quicker methods is possible, these alternatives result in a higher failure rate and lower quality data. Purified plasmid DNA consistently yields the most accurate and reliable sequencing data. By starting with purified plasmid, we significantly reduce the risk of sequencing errors and reaction failures, ensuring that your data is both high-quality and dependable.

For WPS-Express: (< 10 kb)

• Purified Plasmid/RCA Product - Required concentration: 25–100 ng/µL, Volume: >10 µL 

• Overnight Culture/Glycerol Stock - Volume: >30 µL

• Colony Suspension - Pick a single colony, suspend in 20 µL water, Volume: >10 µL 

• Agar Plate - Submit plate with order form and indicate colonies to pick

For WPS-Standard: (< 25 kb)

• High-Copy Plasmid/RCA Product -  Required concentration: 50–100 ng/µL, Volume:  >10 µL 

• Low-Copy Plasmids - Required concentration: As high as possible, Volume: >10 µL 

• Colony Suspension - Pick a single colony, suspend in 20 µL water, Volume: >10 µL 

• Overnight Culture - Volume: >20 µL

For WPS-Pro: (< 25 kb)

• Purified Plasmid DNA - Required concentration: ≥ 200–300 ng/µl, Volume: ≥ 10 µl, Container: 200 µl strip tubes, Buffer: Elution buffer or nuclease-free water 

• Overnight Culture - Buffer: LB, OD600: 2.0–3.0, Volume: ≥ 1.5 ml, Container: 2 ml microfuge tubes

• For WPS-Express service, the turnaround time is next morning for all types of samples upon receipt. 

• For WPS-Standard service, the turnaround time is next morning for plasmid and RCA samples, while bacterial samples require an additional day to process.

Yes, we provide a QC report for WPS-Standard orders. This includes metrics on read length distribution, purity, multimer percentage, mapping statistics, and more. For WPS-Express, QC reports are currently not included.

Yes, for WPS-Standard, we provide raw FASTQ files in the data package. These files are commonly used for downstream analysis and can be processed with various bioinformatics tools. However, for WPS-Express, raw FASTQ files are currently not included in the data package, though we are working on making them available in the future.

If your sequencing results fail or if you contact us because the results did not meet your expectations, we will promptly investigate the cause. If the issue is determined to be from our end, we will update you with our findings and repeat the sequencing at no extra charge. If the cause is related to the samples themselves, you may need to resubmit new samples with a new order.

If you believe there is an issue with your sequencing results, please contact us anytime via email at sales.us@quintarabio.com. We will review your data and investigate potential causes, including sample quality, preparation issues, or sequencing errors.