HT Platform enforced AI antibody drug - Quintara Biosciences — Home of Sequencing. Trusted for Every Base.

Overview

Antibody drug discovery has entered an AI‑empowered era. AI can generate thousands of antibody candidate sequences within hours — yet a sequence remains merely a hypothesis until it is synthesized, expressed, and functionally characterized. Quintara Biosciences bridges this critical divide with one unified, end‑to‑end high‑throughput pipeline.

10,000+ Candidates

10,000+ antibody expression per month
$59/gene

Abgene in 4 business days
From $59/gene
2 Weeks

Purified antibodies in as fast as
2-weeks turnaround
>95% Purity

>95% purity, with a developability data package you can feed back into your model

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Over recent years, artificial intelligence has transitioned from a novelty to a foundational design tool in antibody discovery. Generative and structure-based models generate novel CDR loops, humanize murine leads, optimize affinity, and rescue poor biophysical profiles in silico, condensing months of design effort into days.
AI, however, does not obviate experimentation — it expands experimental workload and raises demands for higher-dimensional, higher-quality data. All high-ranking virtual library candidates require synthesis, cloning, transfection, expression, purification, and characterization to generate empirical data for model retraining. As candidate numbers grow, the limiting factor shifts from computation to wet-lab capacity and data quality.

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One-Stop Solution

True AI‑empowered antibody discovery relies on integrated high‑throughput wet‑lab workflows for high‑quality, robust and representative experimental data. Gene synthesis, plasmid production, mammalian expression, and analytical QC run on our platforms, so an AI-generated sequence entering the pipeline on day one exits as a purified, characterized antibody with a complete data record.

Gene Synthesis

Vector Construction

Plasmid Preparation

Transfection & Culture

Developablity Assay

High-Throughput Gene Synthesis: Abgene

Abgene is Quintara's premium high-throughput clonal gene synthesis solution, purpose-built for AI-driven antibody discovery.

Fast: When your model outputs hundreds or thousands of VH/VL variants, manual PCR, fragment assembly, and colony screening become a 4-day cycle per batch.

Flat: Predictable per-gene pricing makes cost linear with candidate count, so you can afford to synthesize the entire shortlist rather than cherry-picking.

Full-Length: 100% clonal verification option means the sequence your model designed is the sequence you test, make sure your training data stays clean.

Abgene eliminates that entirely: you submit digital sequences and receive 100% sequence-verified clonal genes ready for transfection.

Gene Length	Price/Construct	Turnaround Time
0 - 800bp	$59	4 Business Day
801 - 1,500bp	$109	4 Business Day
• Default vector is pUC57 ; QuinDirect vectors (136 available) • Customer-provided vectors, a $15 vector QC fee applies to each NEW vector. $0 for cloning. Abgene is for simple genes only, and we will evaluate the sequence after receiving your request. If it is a complex gene, the Regular gene service will be another option.

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Clone Vectors

Quintara have mutiple vectors suitable for recombinant antibody, and here are tips for choosing vectors:

Single vs. dual cassette: pcDNA3.4, pTT5, pcDNA3.1 and pCHO1.0 are single-cassette vectors — heavy chain and light chain require two separate plasmids and an optimized co-transfection ratio. pEE12.4 carries both chains on one plasmid, eliminating the ratio optimization step.

Tagged vectors are for fragments only: Avoid Myc-His and Flag vectors for full-length IgG: a C-terminal tag can interfere with Fc conformation, and full-length antibodies are purified via Protein A affinity without any tag.

Signal peptide required: These CMV vectors generally do not carry a secretion signal. An IgG leader sequence must be added to the 5′ end of VH/VL, otherwise the antibody remains intracellular and will not appear in the supernatant.

Vector	Promoter	Suitable Host	Suitable Antibody Type
pcDNA3.4	CMV	HEK293, CHO	Full-length IgG (preferred for transient expression); scFv, Fab, VHH
pTT5	CMV	HEK293 (EBNA1 system)	Full-length IgG (classic high-yield transient vector)
pcDNA3.1(+)	CMV	HEK293, CHO	Full-length IgG; bispecifics (paired with Zeo/Hygro vectors for co-transfection)
pcDNA3.1(−)	CMV	HEK293, CHO	Full-length IgG (reverse MCS; backup or control)
pcDNA3	CMV	HEK293, CHO	Full-length IgG (earlier version; control)
pCHO1.0	CMV	CHO	Full-length IgG (designed for CHO stable lines)
pEE12.4	CMV	CHO (GS system)	Full-length IgG (dual expression cassette — HC + LC on one plasmid); bispecifics
pEE6.4	CMV	CHO (GS system)	Single-chain expression; bispecifics (two plasmids, one chain each)
pcDNA3.1 Hygro(+)	CMV	HEK293, CHO	Full-length IgG (hygromycin selection; stable lines)
pcDNA3.1 Zeo(+)	CMV	HEK293, CHO	Full-length IgG (Zeocin selection; stable lines)
pcDNA3.1 Zeo(−)	CMV	HEK293, CHO	Full-length IgG (Zeocin selection; stable lines)
pCAGGS	CAG	HEK293, CHO	Full-length IgG (alternative when CMV-driven yield is low)
pcDNA3.1 Myc-His A	CMV	HEK293, CHO	scFv, VHH, Fab (C-terminal Myc-His tag)
pcDNA3.1 Myc-His B	CMV	HEK293, CHO	scFv, VHH, Fab (C-terminal Myc-His tag; reading frame B)
pCMV-C-Flag	CMV	HEK293, CHO	scFv, VHH, Fab (C-terminal Flag tag)
pCMV-N-Flag	CMV	HEK293, CHO	scFv, VHH, Fab (N-terminal Flag tag)
p3XFLAG-CMV-14	CMV	HEK293, CHO	scFv, VHH, Fab (C-terminal 3×Flag tag)

High-Throughput Plasmid Preparation

Expression throughput is ultimately capped by DNA throughput: no DNA, no transfection. Quintara's in-house plasmid preparation platform is designed to keep pace with high-throughput synthesis, delivering the quantity and quality transient expression demands. Consistent, sequence-confirmed, high-quality DNA reduces titer variance between candidates — which is the difference between comparing your designs and chasing artifacts.

Scale: Quantity guaranteed, from µg to mg quantities per construct

Continuity: Direct handoff from gene synthesis and cloning — no re-formatting, no re-verification, no shipping delays between vendors

Quality: 100% full-sequence verification available, including Nanopore whole-plasmid sequencing across insert, backbone, and junction regions

QC Items	QC Standards (Transfection Grade)
Appearance	Colorless, clear, free of precipitation or foreign particles
Sequence Accuracy	Sequencing verification matches the order requirements
A260/280	1.8-2.0
A260/230	≥2.0
Supercoiled Content	Supercoiled ≥80%
Residual RNA	Not visible on agarose gel electrophoresis
E. coli Genomic DNA	<15% on agarose gel electrophoresis
Restriction Analysis	DNA bands with expected sizes detected by agarose gel electrophoresis
Endotoxin Level	Endotoxin ≤0.01EU/ug
Bio-Burden Test	No viable microorganisms in/on culture media

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High-Throughput Antibody Expression

Powered by high-throughput recombinant antibody expression platform, candidates are transfected into CHO or HEK293 cells using a proprietary high-expression vector, then cultured and purified at small scale for rapid, comparable readouts.

Scale: Expression scale from 1mL - 50L, from 0.2mg - 50mg.

Fast: Expression turnaround time stars from 5 business days.

Format: IgG, Fab, ScFv, VHH, di-scFV, Bi-specific antibody

Recombinant Antibody

IgGs, scFv, Fab, VHH, di-scFv and more

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Bi-specific Antibody

Asymmetric and symmetric Full antibody and antibody fragments

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FAQ

We already have an AI design team. What do we actually need from you?: Everything downstream of the sequence. Design produces hypotheses. Quintara turns them into purified, measured antibodies at a rate that keeps your model learning rather than waiting.

Can you handle hundreds or thousands of candidates at once?: Yes. Our high-throughput expression platform supports 10,000 projects per month, and Abgene gene synthesis is priced flat per gene specifically to make large candidate panels economical. Contact us to scope campaigns above standard capacity.

My sequences are complex (high GC, repeats, toxic, or very long).: Abgene covers regular antibody genes. Complex or ultra-long constructs up to 200 kb are supported through Quintara Regular Gene Synthesis, delivered in as fast as 3–5 business days.

Can I use my own expression vector?: Yes. In addition to 130+ pre-validated QuinVectors, we accept customer-provided vectors (a vector QC fee applies to each new vector).

What expression system do you use?: Mammalian transient transfection in CHO or HEK293 cells, using Quintara's proprietary high-expression vector — the system of choice for preserving antibody folding, assembly, and PTMs.

Can developability specs be customized?: Yes. Beyond expression process and routine quality QC data, we also offer customizable developability analytics tailored to the requirements of your AI model. Please share your assay requirements with us for further discussion.

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