Quintara Hybrid Microbial WGS combines 30–50× Nanopore long-read coverage with 100× short-read coverage to deliver highly contiguous genome assemblies with improved consensus accuracy. By integrating long-read genome structure resolution with short-read base-level polishing, the workflow is designed for high-confidence reference-quality microbial genome reconstruction. Standard analysis is included with the service, with an optional Methylation Analysis Report available for additional insight into native-DNA methylation patterns.
Combine long reads and short reads for improved genome reconstruction
Capture genome structure and resolve complex regions
Improve consensus accuracy through short-read polishing
Deliver comprehensive assembly, QC, and methylation insights
What Is Hybrid WGS Used For?
Create Build a high-quality genome assembly | Polish Improve consensus accuracy | Produce Generate reference-quality genomes | ||
Generate de novo assemblies without requiring a close reference Construct high-continuity microbial genomes | Combine long reads and short reads for genome polishing Reduce residual base-level errors in assembled genomes | Create highly accurate consensus assemblies Support publication and downstream genome analysis |
An integrated workflow combining Nanopore long reads and short-read sequencing to generate high-continuity genome assemblies with improved consensus accuracy.
Cultured bacterial or yeast isolates
DNA QC and workflow-specific library preparation
30–50× Nanopore long reads combined with 100× NGS short reads
Hybrid de novo assembly and short-read polishing
Sequencing data, polished genome assemblies, and analysis results
Service | Technology & Coverage | Sample Type | Price | Turnaround Time |
Hybrid Microbial WGS | 30–50x Nanopore long-read + 100x NGS short-read | Bacterial | Starting from $165 | 5-10 Business Days |
Yeast | $255 |
* Your project clock starts once we receive your samples.
DNA Extraction — $15 per sample (optional)
Standard Analysis — Free
Methylation Analysis — $25 per sample (optional)
1. Purified gDNA
Concentration: 50 ng/μL
Volume: ≥30 μL
2. Cell Pellet
Volume:1-2mL
Pellet yeast maintained at freezing (dry ice/Ice pack)
Sequencing, Assembly & Polishing QC Report (HTML)
NanoPlot Sequencing QC
Bandage Assembly Visualization
CheckM Completeness & Contamination Assessment
Species Identification
Polished, De Novo Assembled & Annotated Consensus Genome
Raw Nanopore FASTQ Data
Paired-End Short-Read FASTQ Data
Methylation Analysis Report (optional)
Not necessarily. Coverage indicates how many times the genome is represented in the sequencing data, but it does not determine read length or assembly continuity. For de novo assembly, long reads can span repetitive and complex regions that very high-coverage short reads may still fail to connect.
Both workflows are designed for pure bacterial or yeast isolates and require high-quality genomic DNA. Mixed or contaminated samples may affect genome assembly and downstream analysis.
Yes. Organism type and expected genome size help determine sequencing requirements, data yield, and project pricing.
It means the total sequenced bases are roughly 30-50 times the expected genome size on average. Individual regions may receive more or less coverage.
No. Both Long-Read and Hybrid WGS can generate a de novo assembly without relying on a close reference genome. A suitable reference may be used for additional reference-based analyses when needed.
Choose Long-Read when the main goal is de novo assembly and genome structure, Hybrid when both assembly continuity and base-level confidence are important.
Yes. Additional analyses, including reference-based mapping, variant calling, and methylation analysis, may be available depending on project requirements and service availability. Methylation analysis requires native DNA.
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