Name: eGFP mRNA (m1Ψ)
Availability: InStock
Rating: 5.0 (78 reviews)

eGFP mRNA (m1Ψ)

Scale：

0.2 mg 1 mg

Cat. No：

QPM90001-M

Price：

$315.00

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Product Description

This product is an mRNA encoding enhanced Green Fluorescent Protein (eGFP). eGFP originates from the jellyfish Aequorea victoria and is a widely used fluorescent reporter protein for mRNA drug development. It emits intense green fluorescence upon excitation at 488 nm, with an excitation peak at 488 nm and an emission peak at 509 nm.

This mRNA carries a Cap1 (m7GpppNm) structure with high capping efficiency to boost mRNA stability. All uridine (U) residues in the sequence are fully replaced with N1-methylpseudouridine (m¹Ψ), which markedly elevates protein expression and lowers immunogenicity. In addition, the construct contains a poly(A) tail of no less than 100 nt to enhance intracellular mRNA stability and translational efficiency.

This product is for laboratory research use only.

Length	997 nucleotides
Cap	Cap 1
NTP modification	N1-Methylpseudouridine/m1Ψ
Poly (A) Tail	≥100 nt A
Concentration	1.0 mg/mL
Unit scale	200 ug/1 mg

Case Study

eGFP mRNA Expression Assay

To verify the expression efficiency of Quintara’s off-the-shelf mRNA products, we delivered our eGFP mRNA (m1Ψ) into 293T cells via transient transfection. Experimental results demonstrate that the proportion of eGFP-positive cells exceeded 95% at 24 hours post-transfection. Our ready-to-use stock products serve as high-quality standard experimental controls for customers, guaranteeing reliable and reproducible research data.

eGFP mRNA
Bright field

Figure 1. Expression of Quintara eGFP mRNA in 293T cells (24 h post-transfection)

Documents

FAQ

Are your off-the-shelf mRNA products purified? What purification methods are used?: Yes. All of our off-the-shelf mRNA products undergo rigorous purification processes to ensure high purity and quality. Following in vitro transcription, impurities are removed and buffer exchange is performed through Tangential Flow Filtration (TFF) and Oligo(dT) chromatography. The final product is then sterile-filtered and aliquoted for distribution.

How do you control dsRNA residuals in mRNA products to minimize immunogenicity?: We employ an optimized in vitro transcription process designed to minimize dsRNA generation at the source. In addition, downstream purification steps are specifically optimized to further remove residual dsRNA. This combined approach significantly reduces dsRNA levels and minimizes nonspecific immune stimulation in both cell-based and animal studies.

What quality control tests are performed on your off-the-shelf mRNA products?: We implement a comprehensive quality control program, including release testing for RNA size, concentration, purity, pH, and endotoxin levels. Each batch is accompanied by a Report of Analysis (RoA) providing complete QC results.

How is mRNA integrity evaluated and quantified?: mRNA integrity is assessed and quantified using automated capillary electrophoresis. The integrity profile and analytical results are documented in the Report of Analysis (RoA) provided with each batch.

Where can I find information about mRNA sequence length, capping method, and nucleotide modification: Detailed product specifications, including sequence length, capping technology, and nucleotide modifications, can be found in the corresponding technical datasheet or product documentation. You may download these documents from the product page on our website. For additional information, please contact our sales or technical support team at sales.us@quintarabio.com.

Do you offer custom services if I cannot find an off-the-shelf product that meets my requirements?: Yes. In addition to our catalog products, Quintara provides fully customized mRNA synthesis and LNP encapsulation services. Customers may specify sequence design, nucleotide modifications, capping strategies, lipid formulations, and other project-specific requirements. Please contact our sales or technical support team at sales.us@quintarabio.com for further assistance.

What is the remaining shelf life of off-the-shelf mRNA products upon shipment?: To ensure product quality and maximize usability, we guarantee that all products have a minimum remaining shelf life of six months at the time of shipment. Manufacturing date and expiration information are provided in the accompanying Report of Analysis (RoA) and on the product label. Products should be stored at ≤ -70°C.

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eGFP mRNA (m1Ψ)