Choosing the Right WAS Approach

• Research Objective

Confirm that a cloned construct contains the correct insert sequence without unintended mutations before downstream experiments such as protein expression, viral vector production, or genome engineering.

• Recommended Service

1.  Sanger Sequencing

Choose Sanger Sequencing for short, clean, single-template amplicons and routine local verification. It is the preferred method when the goal is to confirm a simple point mutation, junction, or insert region within a practical read length, providing fast and reliable validation of cloned constructs.

2.  Nanopore Sequencing

Choose Nanopore Sequencing when full-length amplicon analysis is required. Long-read sequencing enables comprehensive characterization of the entire PCR product in a single read, making it suitable for complex amplicons, primer-independent verification, and high-throughput screening workflows.

• Research Objective

To determine the complete outcomes of genome editing by identifying and quantifying different allele variants, including knock-in, knock-out, and complex editing patterns across the target region.

• Recommended Service

1.  Nanopore Sequencing

Choose Nanopore Sequencing when full-length allele resolution is required. Long-read sequencing enables direct characterization of complete amplicon structures, allowing accurate identification of knock-in/knock-out outcomes, complex indel patterns, and mixed editing populations.

Example application: In CRISPR-edited mosaic cell populations

Nanopore single-molecule sequencing directly reads individual DNA molecules to distinguish diverse editing products, estimates product frequency based on read counts, and provides full-length coverage to span large fragment variations, enable haplotype linkage analysis, and reveal the linkage state of mutations.

2.  Illumina NGS

Choose NGS when precise quantification of editing outcomes is the primary goal. High-depth sequencing enables accurate measurement of edit frequencies, variant abundance, and SNP/indel distributions, making it suitable for short targeted loci and large-scale screening workflows.

• Research Objective

To profile microbial diversity, detect targeted genetic variations, and characterize specific genotypes from complex environmental or biological samples.

•  Recommended Service

1.  Illumina NGS

Choose NGS Sequencing when high-depth profiling and quantitative analysis are required. Targeted sequencing enables accurate characterization of microbial communities, targeted pathogens, immune repertoires, and barcode distributions.

Example applications: 

- 16S / ITS Microbiome: Taxonomic profiling, community composition, relative abundance, and group-to-group comparison using targeted amplicons.

- Targeted Pathogen / Strain ID: Species detection, strain differentiation, and surveillance assays for defined microbial targets.

- TCR / BCR & Barcode Counting: Clonotype diversity, immune repertoire dynamics, engineered construct barcodes, and variant counting.