Quintara | Genotyping & Gene-Edit Analysis

Overview

Genotyping Validation is a full-length amplicon sequencing service for targeted genotyping of single-clone samples. Designed for PCR amplicons from 100 bp to 25 kb, the service provides full-length allele sequences to identify WT and edited alleles, characterize indels and other variants, and resolve complex genotypes through long-read sequencing. Both purified and unpurified PCR products are accepted, with cleanup performed before library preparation. The service is intended for single-clone validation and is not designed for pooled or bulk-edited populations.

Launch Promotion

Offer:
Genotyping Validation - Up to 50% OFF >> Starting from $5/Sample, with only $1 Clean-up Fee (Promo Code: LaunchGenotyping)
Terms and Conditions:
• The promotion applies to all qualifying orders valid Sep 1 through Sep 30, based on ET.
• The discount will be applied automatically when you place your order through the QuinGo online portal.
• Quintara Biosciences reserves all rights to this promotion.

Full-Length Alleles
Precise Genotyping
Complex Edit Detection
Allele Phasing

What Is Genotyping Validation Used For?

Gene-Edit Clone Validation		Complex Genotype Resolution		Full-Length Variant Phasing		Genotype-Based Sample Stratification
Confirm the genotype of gene-edited clones and identify WT and edited allele sequences.		Resolve multiple allele sequences in cancer cells, polyploid or aneuploid samples, and amplified loci.		Link SNPs, indels, insertions, deletions, and multiple edits to specific full-length allele sequences.		Classify samples by resolved genotype or allele sequence for downstream genotype–phenotype analysis.

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Workflow

Our genotyping sequencing service accepts customer‑supplied PCR products, both purified and unpurified, for downstream processing. Samples undergo library preparation and long‑read sequencing to obtain full‑length sequence information. The bioinformatics pipeline clusters haplotypes and applies built‑in filtering rules to deliver final allele‑level genotyping results.

Sample Submission

Accept customer PCR amplicons
Library Preparation

Build long‑read sequencing libraries
Long-Read Sequencing

Generate high‑quality raw reads
Allele & Genotype Analysis

Haplotype clustering & variant calling

Services

Service	Size	Read Target	Alleles Reported	Sample Type	Price⁺	Turnaround Time*
Basic	100bp-25kb	Up to 1K reads	Up to 3	Unpurified PCR Product; Purified PCR Product	$10 $5	1 Day
Standard		Up to 4K reads	Up to 6		$30 $25	1 Day
Plus		Up to 8K reads	Up to 12		$60 $55	1 Day
Huge		Up to 16K reads	Up to 24		$120 $115	1 Day

*Your project clock starts once we receive your samples.

+ Promotion Price

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Service Specifications

Sample Input

Unpurified PCR product (10μL, 2ng/μL per 100bp)
Purified PCR product (10μL, 2ng/μL per 100bp)
Deliverables

1. Allele/Variant FASTA Files
2. Allele/Variant Frequency File
3. Raw Data FASTQ File
4. Alignment File
5. Interactive QC Viewer
6. Allele Per-Base Data File

FAQ

How is a reported allele defined in this service?

A reported allele is a unique full‑length amplicon haplotype reconstructed from clustered sequencing reads, representing one sequence variant at the target locus.

Important note: Reported alleles do not map one‑to‑one to inherited chromosomal copies. Multiple genomic copies with identical sequences will collapse into one single reported allele. The number of reported alleles cannot be used to infer genomic copy number.

What is the five‑percent reporting cutoff?

This service applies a 5% reporting cutoff. Haplotypes require ≥5% of supported reads to become eligible for reporting.

Important note: Passing the 5% threshold is only one eligibility criterion. Final output is further constrained by maximum allele tier‑limit and QC filters. Meeting 5% does not guarantee reporting. Real low‑abundance alleles below 5% read fraction will be omitted even if present in raw sequencing data. Reported frequencies are approximate read‑based fractions. PCR read fractions cannot be used to infer genomic copy number.

What factors may cause allele dropout or spurious alleles?: Alleles may be missed if primer‑binding sites are deleted and target fails to amplify. Non‑specific PCR products may be mis‑identified as spurious alleles. Only variants within the submitted amplicon are analyzed.

What results are delivered, and what analysis is not supported?: Deliverables include full‑length allele sequences, estimated allele frequencies (%), and supporting read counts. Absolute genomic copy‑number calling is not supported. Alleles below 5% read fraction are not reported.

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