mRNA & LNP One‑Stop Solution｜IVT Plasmid & Linear Template Service

Overview

From sequence design and gene synthesis to IVT mRNA production and LNP encapsulation, Quintara brings the key stages of mRNA development together in one integrated workflow. Whether you start with a protein coding sequence, or an mRNA sequence, our flexible service portfolio accelerates your project from starting sequence to finished mRNA or mRNA-LNP. A single integrated partner eliminates multiple supplier handoffs and reduces coordination burden. With sequence design, template build, mRNA synthesis and LNP encapsulation managed together, standardized technical oversight and QC mitigate compatibility risks, simplify project management, and deliver greater workflow consistency.

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Services

Sequence Optimization

Optimize coding sequences and key sequence elements for efficient IVT transcription and mRNA quality.

IVT Compatibility - Evaluate sequence features relevant to T7 transcription.
Expression Optimization - Design with downstream protein expression in mind.
Risk Screening - Review GC, repeats, structure, and sequence risks.

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Have a protein or coding sequence? → Optimize it for IVT & expression.
Gene Synthesis and Cloning

Build sequence-verified plasmids with optimized mRNA elements and an IVT-ready vector architecture.

Quintara Optimized UTRs - Proprietary 5′ and 3′ UTR designs.
Flexible Poly(A) Architecture - 60A and 100A configurations available.
IVT-Ready Vector Design - Integrated T7 promoter and defined linearization sites.

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Have an optimized sequence? → Build the plasmid template.

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Plasmid Prep and Linearization

Prepare, purify, and precisely linearize plasmid DNA to create a clean, consistent transcription template.

High-Purity DNA - Clean and consistent plasmid preparation.
Complete Linearization - Verified conversion to the intended linear template.
Template Integrity - Intact DNA with controlled quality for consistent production.

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Have a plasmid? → Prepare and linearize it for IVT.

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Poly(A) Guarantee

Validated plasmid architecture designed to maintain long Poly(A) sequences during cloning and plasmid production.

Validated Vector Architecture – Maintains long Poly(A) sequences with minimal truncation.
Stable Plasmid Propagation – Reduces Poly(A) instability during cloning and amplification.
Consistent IVT Template – Uniform DNA templates for reproducible mRNA synthesis.

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Need long-encoded Poly(A)? → Choose a vector designed for stability.
IVT mRNA Synthesis

Generate research-ready mRNA with flexible specifications, comprehensive QC, and ready-to-use product options.

Custom IVT – Tailored cap, poly(A), nucleotide, and sequence design.
Strict QC – Verify mRNA integrity, purity, identity, and key product attributes.
Off-the-Shelf - Ready-made mRNA products for rapid experimental initiation

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Need mRNA? → Choose custom production or ready-to-use products.

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LNP Encapsulation

Formulate purified mRNA into characterized LNPs with flexible lipid options and optimized encapsulation conditions.

Flexible Lipids - Validated formulations and customer-supplied lipid options.
FTO-Ready Options - Formulations selected to support research with freedom-to-operate considerations.
Strict QC - Characterize particle size, PDI, encapsulation efficiency, and key formulation attributes.

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Need LNP encapsulation? → Formulate it into LNPs for delivery studies.

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Workflow

From sequence design to mRNA-LNP, our integrated workflow brings together gene synthesis, DNA template preparation, mRNA synthesis, and LNP encapsulation—streamlining the path from your sequence to your final RNA product.

1

Sequence

Optimization
2

Gene

Synthesis & Cloning
3

Plasmid

Prep & Linearization
4

Poly(A)

Verification
5

mRNA

IVT & QC
6

LNP

Encapsulation

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Case Study

Case Study 1: Cap-Driven Sequence Optimization

Scientific Background

T7 RNA polymerase generally favors G‑rich sequences at the transcription initiation site, making GGG a commonly used initiation sequence for IVT mRNA synthesis. However, the optimal initiation sequence cannot be determined by transcription efficiency alone. When co‑transcriptional capping is incorporated into the IVT process, the initiation sequence must also be considered in relation to the selected capping chemistry and its mechanism of transcription initiation.

Project Summary

The customer selected our partner‑supplied AG‑type Cap1 structure as an IP‑conscious capping option for their mRNA production. To improve template compatibility with the selected capping workflow, we evaluated the 5′ initiation region and optimized the initiation sequence from GGG to GAG, while preserving the intended coding sequence.

This process‑specific template engineering is designed to align the construct with the AG‑type Cap1 system. The design objectives include improved capping efficiency and reduced formation of 5′‑triphosphorylated impurities, together with robust transcription initiation and controlled 5′‑end formation within the chosen production workflow.

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Fig. Schematic of Template Design Before and After Initiation Motif Engineering

Case Study 2: Proprietary mRNA-Optimized Vector Platform

Scientific Background

Conventional gene synthesis typically focuses on accurate DNA sequence construction and cloning, without necessarily considering the sequence and architectural requirements of IVT-mRNA production. An mRNA-focused vector platform can integrate essential elements—including optimized UTRs, a validated Kozak sequence, a defined Poly(A) sequence, and strategically positioned linearization sites—into a standardized template architecture. This provides a consistent foundation for efficient IVT template construction across different coding sequences.

Project Summary

We developed proprietary mRNA-focused vectors specifically designed for IVT-mRNA template construction. The vector architecture incorporates validated UTR elements, a validated Kozak sequence, and a defined 100–120 bp Poly(A) sequence to support mRNA stability and translation. For each CDS, sequence optimization can include GC content and codon adaptation, while predicted secondary structures and unwanted restriction sites are evaluated during construct design. A unique IIS restriction site downstream of the Poly(A) region enables precise linearization of the plasmid to generate the intended transcription template.

By integrating mRNA-specific sequence elements with a standardized vector architecture, Quintara provides a consistent framework for IVT template construction while maintaining flexibility for different coding sequences and mRNA designs.

Case Study 3: Off-the-Shelf eGFP mRNA Expression Assay

Scientific Background

mRNA-based expression systems are widely used to evaluate RNA stability, translation, and intracellular activity. eGFP, originally derived from Aequorea victoria, is a well-established fluorescent reporter for monitoring mRNA expression. Key mRNA features—including Cap1, N1-methylpseudouridine (m¹Ψ), and a defined poly(A) tail—can support mRNA stability, translation efficiency, and reduced innate immune recognition, making eGFP mRNA a useful model for RNA research and development.

Project Summary

To verify the expression efficiency of Quintara’s off-the-shelf mRNA products, we delivered our eGFP mRNA (m1Ψ) into 293T cells via transient transfection. Experimental results demonstrate that the proportion of eGFP-positive cells exceeded 95% at 24 hours post-transfection. Our ready-to-use stock products serve as high-quality standard experimental controls for customers, guaranteeing reliable and reproducible research data.