Overnight Sanger Sequencing Service

Overview

Quintara Biosciences provides fast, cost-effective Next Generation Sequencing (NGS) services using cutting-edge platforms. We offer a range of solutions, including genome sequencing, single-cell sequencing, 16S/ITS sequencing, and amplicon sequencing.

Cutting-edge platform
Fast, economical and efficient
Next Generation Sequencing service
We offer a range of solutions

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Workflow

01

Sample Submission

02

Library Preparation

03

Sequencing

04

Data Delivery

Services

DNA Sequencing: We support a wide variety of starting materials for DNA sequencing, including tissue, cells, plasmids, and genomic DNA. Our services, tailored to your sample type, include nucleic acid extraction, PCR, and indexing to ensure exceptional data quality. Results are available in as little as three business days. Results are available in as fast as 3 business days.

AmpExpress
Amplicon Sequencing Regular
Amplicon Sequencing 16S/ITS Microbiome
Amplicon Sequencing Genomic DNA
Amplicon Sequencing

AmpExpress sequencing provides a fast, dependable solution for purified PCR products ranging from 150 to 500 bp. Each sample generates up to 40K paired-end reads at 250 bp per read. With a rapid 3-business-day turnaround and pricing starting at just $60 per sample, AmpExpress delivers high-quality results and exceptional value for your research.

Promotion Code:
AmpNGS
Starting Materials:
purified PCR product with adapter (without +$30)
General Primer:
Forward primer with R1 adapter: 5ʼ TCCCTACACGACGCTCTTCCGATCTNNNNNN(followed by your forward primer sequence)
Reverse primer with R2 adapter: 5 ʼGTTCAGACGTGTGCTCTTCCGATCTNNNNNN(followed by your reverse primer sequence)

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Depending on your sample type, we provide nucleic acid extraction, PCR, and indexing to ensure optimal results. With flexible sequencing options and quick turnaround times. Pricing starts at $60 per sample.
Standard Sample Type:
Mouse tissue, Cell pellets, Bacteria culture, Plasmid DNA, genomic DNA
Standard Volume:
20 μL DNA at 15-50 ng/ul, or 250,000 cells, or 1 g tissue or 1 g bacteria culture
Primers:
Please provide at least 20uL of PCR primers at 10pmol, or submit the PCR primer sequence, and we can synthesize it for you.

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16S/ITS rRNA sequencing is a precise technique for identifying and classifying microbial communities. It allows researchers to characterize microbial populations, evaluate species diversity, and investigate community structure in diverse environments.
Standard Sample Type:
Environmental samples (e.g., soil, water), clinical samples, or microbial cultures, DNA extracted from above samples
Standard Volume:
10-50 ng of total DNA

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Our Genomic DNA sequencing service is perfect for whole-genome sequencing of both large genomes, such as human and mouse, and smaller genomes like bacteria and viruses. FASTQ files. For data analysis, we provide comprehensive solutions, including variant detection and genomic profiling.
Standard Sample Type:
Mouse tissue, Cell pellets, Bacteria culture, Plasmid DNA, Genomic DNA
Standard Volume:
20 μL DNA at 15-50 ng/ul, or 250,000 cells, or 1 g tissue or 1 g bacteria culture

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RNA Sequencing: Need to study gene expression or identify non-coding RNA linked to your disease model in just one week? Try our RNA sequencing service. We guarantee mRNA (poly-A selection) or total RNA (rRNA depletion) sequencing data within 7 business days.

Bulk
mRNA Sequencing Whole Transcriptomics
Sequencing

We use poly-A selection to isolate mature mRNA transcripts, which contain poly-A tails. This technique enriches for mRNA, making it ideal for capturing coding RNA sequences and providing an accurate representation of gene expression.
Standard Sample Type:
Mouse tissue, Cell pellets, Purified RNA
Standard Volume:
50 μL RNA at 100 ng/ μL, RIN＞7 or 250,000 cells, or 1 gram tissue

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We apply rRNA depletion to selectively remove rRNA, enriching the remaining RNA for other transcripts. This approach is ideal for capturing a wider range of RNA species, providing a more complete view of the transcriptome.
Standard Sample Type:
Mouse tissue, Cell pellets, Purified RNA
Standard Volume:
50 μL RNA at 100 ng/ μL, RIN＞7 or 250,000 cells, or 1 gram tissue

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Single Cell Sequencing: 10x Single Cell
Gene Expression Sequencing Visium HD Spatial
Profiling

10x Single Cell Gene Expression Sequencing enables gene expression profiling at the individual cell level. This technique uncovers cellular heterogeneity within complex tissues, providing deeper insights into gene expression patterns at the single-cell resolution.
Standard Sample Type:
Fresh or cryopreserved single-cell suspensions
Standard Cell Number:
500-10,000 cells per sample

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Visium HD Spatial RNA Sequencing integrates high-throughput RNA sequencing with spatially resolved gene expression analysis, allowing researchers to map gene activity directly to specific tissue regions. We use poly-A selection to isolate mature mRNA transcripts, which contain poly-A tails. This technique enriches for mRNA, making it ideal for capturing coding RNA sequences and providing an accurate representation of gene expression. Results delivered in 7-14 business days, starting at $30.
Standard Sample Type:
Fresh-frozen, Fixed-frozen or FFPE (formalin-fixed paraffin-embedded) tissue sections
Standard Tissue Section Thickness:
3-10 μm

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Data Analysis: Amplicon SNP/Indel Analysis
Amplicon Sequencing Abundance Analysis
16S/ITS Microbiome Analysis
Differential Gene Expression Analysis
Large Genome Mapping and Variant Calling
Small Genome Mapping and Variant Calling
VDJ Analysis