In AAV gene therapy, everything starts with the plasmid — and everything breaks at the ITRs. ITR deletions, rearrangements, or point mutations silently erode packaging efficiency, skew transgene expression, and can trigger CMC deficiencies during IND review. Even minor ITR deletions or rearrangements can reduce viral yield, alter tropism, and compromise reproducibility.
We've engineered a four-layer defense — specialized host strain, validated vector backbones, dual ITR sequencing, and propority plasmid preparation process to deliver research-grade through preclinical-grade AAV plasmids you can actually build on.
High-fidelity gene synthesis with 99.9% project delivery reliability, backed by 20+ years of gene synthesis experience.
Custom cis-plasmid (GOI + ITRs)
ssAAV / scAAV frameworks; ITR145 / ITR141 / ITR128 variants
As fast as 5 business days
A proprietary ITR-targeted Sanger method reads through both hairpin regions; Nanopore long-read confirms ITR-to-ITR clonal homogeneity.
Detects partial/full deletions, point mutations & recombinants
Every qualifying batch ships with an ITR verification report
Trace files, coverage maps & clonal homogeneity call included
Mini- to large-scale AAV plasmid DNA from GenStable™ ITR-optimized E. coli, with supercoiled enrichment and controlled endotoxin.
Endotoxin < 0.1 EU/µg (research / RUO / GLP tiers)
Transfection-grade
Custom transfer plasmids as fast as 3 business days
Proprietary AAV Applied Vectors Pre-configured vectors with built-in ITR sequences for reliable downstream packaging and seamless research applications.
Ampicillin / Kanamycin dual resistance; smaller clone, higher yield
Drop-in replacement for pSub201-class legacy backbones
Insert GOI and proceed directly to packaging
A linear, transparent five-step process. Each step is a service we provide; together they form one traceable chain from your design to a release-ready AAV plasmid.
Custom cis plasmid synthesis, and cloning into AAV backbones
A single ITR-verified colony is isolated and used for all downstream preps.
Mini to gram-scale, research /transfection tiers available.
Proprietary Sanger + Nanopore long-read ITR confirmation
Challenge | Standard Workflow | Our AAV Plasmid Solution |
ITR sequencing through hairpins | Often incomplete / early termination | Proprietary ITR Sanger — full hairpin coverage |
Detecting rare ITR recombinants | Missed by short-read methods | Nanopore long-read ITR-to-ITR detection |
ITR loss during bacterial passaging | Common in standard hosts | Proprietary host suppresses ITR deletion |
Plasmid topology for transfection | Variable supercoiled fraction | Supercoiled-enriched, endotoxin-controlled prep |
Release criterion | ITR verification often optional | Dual ITR QC is a release gate, not an add-on |
ITRs drive AAV replication and encapsidation — yet their high-GC, palindromic structure makes them unstable in standard E. coli hosts and difficult to read by conventional Sanger sequencing.
Scenario | What Goes Wrong | What We Guarantee |
Viral packaging | Replication stalls, empty capsids rise, titer drops 1–2 logs | Dual-sequencing rejects mutant clones before release |
In vivo expression | Promoter truncation, transgene silencing | Nanopore confirms full expression cassette end-to-end |
Regulatory readiness | CMC homogeneity fails, regulatory deficiency letter | RUO/GLP-grade ITR certificate with every qualifying batch |
Reproducibility | Batch-to-batch drift kills repeatability | GenStable host suppresses ITR loss across passages |
Accelerate research with Quintara’s accurate, reliable Sanger Sequencing overnight.
Discover more
Precision and custom gene synthesis from Quintara Biosciences for your research needs.
Discover more
Accelerate research with Quintara’s accurate, reliable Sanger Sequencing overnight.
Discover more
We Go Fast, You Go Forward
sales.us@quintarabio.com
1-617-789-6111
3563 Investment Blvd, #2, Hayward, CA 94545sales.us@quintarabio.com1-617-789-611165 Grove St, Suite 102, Watertown, MA 02472sales.us@quintarabio.com1-617-789-611110410 Corporate Dr. Suite 118, Sugar Land, TX 77478sales.us@quintarabio.com
1-424-549-091110601 Virginia Avenue, Culver City, CA 90232sales.us@quintarabio.com1-619-823-08956364 Ferris Square, Suite 102, San Diego, CA 92121Quintara Biosciences © 2025 All Rights Reserved | Site Map | Terms of Use | Privacy Policy
Login 
Order
Contact Us
Contact Us
