IVT mRNA Synthesis

Your mRNA is synthesized via in vitro transcription (IVT) and supplied as a liquid solution, shipped on dry ice. Upon arrival, the mRNA may appear as a white pellet or concentrate at the bottom of the tube.

For first use:

1. Thaw the mRNA on ice.

2. Briefly centrifuge the tube before opening.

3. Aliquot the solution into single -use portions to avoid repeated freeze-thaw cycles.

4. Use only certified RNase -free reagents, tubes, and tips, and maintain RNase -free techniques throughout.

For storage after aliquoting:

l   Store at ≤ -70°C for long-term stability.

l   Avoid repeated thawing and freezing, as this can degrade mRNA integrity.

The mRNA supplied is suitable for most standard in vitro cell transfection experiments. It can be delivered using a variety of commercially available transfection reagents designed specifically for mRNA, according to manufacturer protocols.

Best practices for RNA work:

l   If possible, designate a clean, separate area for RNA handling.

l   Before starting, sanitize your bench surface and pipettes with RNase inactivating agents.

l  Always wear clean gloves and use RNase -free consumables.

Upon receipt, store mRNA at ≤ -70°C. Minimize freeze-thaw cycles to maintain product stability and performance.