Accelerate your research with Quintara’s Sanger Sequencing services. We specialize in delivering accurate and reliable DNA sequencing results with an industry-leading overnight turnaround times. Our service is designed for efficiency and supports a wide range of applications, including plasmid verification and mutation analysis, ensuring your projects move forward swiftly without sacrificing quality.
Accelerate your research
Industry-leading overnight turnaround
Improve efficiency
Supports a wide range of applications
| Name | Description | Turnaround* | Price |
| Sanger Sequencing | Samples can be mixed or non-mixed with primers | Overnight | Starting from $4 |
| Yeast Colony Sequencing | Direct colony sequencing | Overnight | Starting from $15 |
| Value-Added Services | We provide specialized purification and preparation services tailored to your starting material. ✓ PCR Amplification & Clean-Up: We'll amplify and purify your PCR reactions from raw product. ✓ RCA Clean-Up: We specialize in purifying Rolling Circle Amplification products. ✓ Plasmid Mini-preps: Get pure plasmid DNA directly from bacterial cultures. Our services accept a range of sample types, including unpurified PCR products, Genomic DNA, RCA products, and bacterial cultures | / | Starting from $1 |
* Your project clock starts once we receive your samples.
| Plasmid DNA Template | 5 μL Primer1 | |
| Volume | Concentration | Concentration |
| 10- 15 μL | 80 ng/ μL | 5 pmol/μL (5 μM) |
2. Purified/Unpurified PCR DNA and Primer in Separate Tubes, or Use Our Free Primer Library. (Complete List in Excel or Search Online)
3. Please provide the PCR product size information at the time of order submission
| Purified PCR | 5 μL Primer2 | |
| Type & Size | Concentration | Concentration |
| ＜ 1 Kb | 10 ng/ μL | 5 pmol/μL (5 μM) |
| 1 kb – 2 kb | 20 ng/ μL | |
| 2 kb – 3 kb | 30 ng/ μL | |
| 3 kb – 4 kb | 40 ng/ μL | |
| >4kb | ||
1In the case of unpurified PCR samples, we will conduct a PCR purification process prior to sequencing
2Primer For Sequencing: Prefer Tm: 50-60℃, GC content: 40-60%
agar plates, colony suspensions or overnight cultures
DNA Amplification Methods:
1. PCR (Preferred): This method allows for next-day data delivery. Please provide the amplicon size, as well as forward and reverse PCR primers.
2. Rolling Circle Amplification (RCA).
3. Plasmid Miniprep: Include antibiotic information for the sample.
Note: RCA and plasmid miniprep methods may extend the data delivery timeline by an additional day.
Ensure all suspension samples are placed in either tightly sealed 8-strip tubes or in a strip-capped 96-well plate
1. PCR amplification prior to sequencing. Please provide the PCR primer and the Expected Amplicon Size.
2. A minimum of 10 μL DNA Sample with a concentration of at least 30 ng/μL.
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Quintara Biosciences offers rapid, cost-effective, full range of genomics services, including DNA.
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