Quintara Biosciences

Primer Walking

QuintaraBio’s Primer Walking service is designed to sequence an entire plasmid or a large portion of DNA greater than 1kb. Our procedure sequences individual segments of DNA using separate primers, these segments are then connected by overlapping the ends of each fragment, around 100 bp for each overlap is enough to confidently deem as one continuous strand of DNA.

Given that most of the sequence of the DNA strand is unknown, using the sequence of a known starting point, we design a primer that will follow and read the next segment, a process that is repeated as many times as needed to provide a long and accurate read.