QuintaraBio has been providing the highest quality Sanger DNA Sequencing services to the life science research community since 2005. Our mission as the leading service provider is to ensure excellent customer service, fast turnaround time, advanced technical support and offer the best value to our customers. 

SimpliSeq^TM DNA Sequencing is the most commonly requested service, for plasmid DNA, PCR fragment, phage DNA, with primer premixed or unmixed.

DirectSeq^TM Colony Sequencing is the best one-stop service for customers that prefer to minimize the risk of low copy number or purification quality. Just send us your bacteria colonies on agar plates or in liquid culture, and expect your data delivered to your email account within 24 hours.

QuintSeq^TM On-demand Sequencing is our premium service designed to expedite the process and shorten your research cycle. After sequencing the bacterial colonies and delivering the data, we keep your colonies and plasmid for one month. During this period, we’ll send back the colony or purified plasmid based on your request without additional charge.

Benefits

• The most flexible sequencing service with the highest success rate.
• Skilled analysis of your sequencing results.
• Expert troubleshooting to optimize more complex samples (hairpin, GC-rich templates).
• Additional services (template amplification, PCR purification) are included in the service package.
• Optional sequence editing and sequence alignment are available free of charge.

Quality

• Average read lengths: 700-1000 bp
• Color printouts of chromatographs are provided free of charge upon request.
• Optional sequence alignment saves time when analyzing sequencing results.
• Sequence editing is provided upon request. (Sequence editing means a manual proofread performed by an experienced staff to correct mistakes /uncertainties in sequences generated by the automatic base caller)
• Troubleshooting: We propose further troubleshooting steps based on the sequencing results. It is your decision whether we continue sequencing your samples.
• Repeat policy: Failed samples will be repeated based on our interpretation of your sequencing results. The repeat is free of charge.

• Plasmid DNA purified by commercially available kits
• Low copy number plasmids need to be further concentrated by spin column, ETOH precipitation, or RCA before sequencing
• Check quality and concentration of plasmid by gel electrophoresis and A260/A280

• PCR products need to be purified to eliminate primers and dNTPs
• Check quality and concentration after purification by gel electrophoresis (2ul) confirming the presence of one specific band
• For unpurified PCR samples, please mark as “unpurified PCR”

• Colonies need to grow for at least 16 hours at 37°C to reach a good visible size
• Place all samples in either tightly sealed 8-strip tubes or strip capped 96-well plate