More than PCR,
so you truly KNOW their genotypes
Combine PCR with cutting-edge sequencing technology to genotype your samples. g-KNOW-typing provides the data you need at a pace and depth you have never experienced before!
Fast 24-hour turnaround upon receiving samples
Uses the same PCR/gel assay that is used in your lab, easy to troubleshoot and compare.
Know the exact sequence of your targets, not just the size
Digital gel simulation for clear result presentation
Investigate any "non-specific" product
One-top solution for identifying mutations
FREE daily sample pickup is provided with local courier service in MA, CA, CO, CT, MD, TX, and more.
Email order@quintarabio.com to order.
WORKFLOW
Target Specific PCR
Barcoding PCR
Pooling / Cleanup
Sequencing
Data Analysis / Report
What do you get in the report
How Can g-KNOW-typing Help
Scenario 1: My WT and TG targets are the same or very similar in size.
We will provide complete sequence composition of the targets. With reference sequences, we can easily distinguish mutants from WTs.
Scenario 2: There are always non-specific targets in my genotyping PCR, Scenario 2: masking the "real" bands.
We use the reference sequence to identify the desired target(s), and can selectively plot them on a simulated gel. Thus, you don't have to worry about the non-specific amplicons.
Scenario 3: A "non-specific" or unknown target showed up in my genotyping Scenario 3: PCR, should I be concerned about it?
We can include PCR products at specific sizes in the analysis, and provide their exact sequence composition, so you know their exact identity.
Scenario 4: We are screening a new mouse line. We have to make sure the mutation is correct as well as the sequences of homology arms, Scenario 4: and we have to do it within a week.
g-KNOW-typing is perfect for such applications! We can plan assays with you and have oligos synthesized ahead of time. Once we receive the tissues, we will do the PCR screening AND provide sequence information from each pup.